Testing Effect Of Immunomodulator Ethanol Extract Of Mengkudu Leaves (Morinda Citrifolia) On Macrofag Cell Activities In Mencit Balb/C Infected Plasmodium Berghei

Main Article Content

Jon Farizal
Leni Marlina
Susiwati Susiwati
zamharira Muslim


Background malaria fever is a disease that is still a problem in developing countries. Plasmodium bergheiis a facultative intracellular parasite, so the immune system that plays a role is the cellular system. Morinda citrifolia is a traditional medicinal plant that contains many active compounds that can reduce the number of malaria parasitemia. Proving the immunomodulatory effect of ethanol extract ofmorinda citrifolia on phagocytosis activity of Balb/c mice infected with Plasmodium berghei. The data were obtained from calculating the number of phagocytosis activity of macrophage cells in each field of view. A different test for macrophage phagocytosis using One Way ANOVA followed by Post Hoct Test. Method this type of research is experimental with the design of the post-test only control group design in experimental animals balb/c mice consisting of 24 male mice, divided into 4 groups. (K) is the control group given Aquabidest, and the treatment group (P1, P2, P3) which was given extract morinda citrifolia with stratified doses (0.64 mg / kgBb / day, 1.28 mg / kgBb / day, 2.56 mg / kgBb / day). The treatment process was given for 13 days and on the 6th day was infected with Plasmodium bergheias much as 0.1 ml x 106 intraperitoneally. On the 14th day, peritoneal fluid isolation was carried out followed by an examination of macrophage cell phagocytosis activity. Result ethanol extract Morinda citrifolia can increase macrophage phagocytosis, between control and treatment P1, P2, and P3 have significant differences, but between treatments, there is no significant difference. Conclusion. Ethanol extract of Morinda citrifolia in various doses can increase the phagocytosis of macrophages in Babl/c mice infected with Plasmodium berghei.

Article Details

How to Cite
Farizal J, Marlina L, Susiwati S, Muslim zamharira. Testing Effect Of Immunomodulator Ethanol Extract Of Mengkudu Leaves (Morinda Citrifolia) On Macrofag Cell Activities In Mencit Balb/C Infected Plasmodium Berghei. SANITAS [Internet]. 4Jan.2021 [cited 23Jan.2021];11(2):246-55. Available from: https://sanitas.e-journal.id/index.php/SANITAS/article/view/20201201-farizal
Medical Laboratory


World Health Organization. World Malaria Report 2015. Avenue Appia; 2015.

Kemenkes RI. profil Kesehatan Indonesia. Vol. 70, Kesehatan. 2016. 1780-1790 p.Available from: http://www.depkes.go.id

R. F. The incidence of environment related illnesses in North Bengkulu, Indonesia. J Environ Health [Internet]. 1994;57(1):16–8. Available from: http://ovidsp.ovid.com

Prevention C for DC and. Anopheles Mosquitoes. 2015;24(7):1–14.

Innocent O, Oke O, Anthony O. Plasmodium berghei Malarial Infection Reduces Blood And Brain Glucose Levels In Experimental Mice . 2013;1(1):1–7.

Soe UTM, Mya D, Chaw N, Naw D, Myint H. Larvicidal efficacy of Morinda citrifolia L. leaf extract against three important mosquitoes. 2015;(5).

Rethinam P, Pratap UP. Pharmacological properties and clinical applications of Morinda citrifolia L . 2015;10:1–18.

Weekly LS. Patents ; Patent Application Titled " Method and Composition for Administering Bioactive Compounds Derived from Morinda Citrifolia ". 2013;1–3.

Puti I, Sabirin R, Maskoen AM, Hernowo BS. Peran Ekstrak Etanol Topikal Daun Mengkudu ( Morinda citrifolia L .) pada Penyembuhan Luka Ditinjau dari Imunoekspresi CD34 dan Kolagen pada Tikus Galur Wistar Role of Noni (Morinda citrifolia L .) Leaf Ethanolic Extract Topical Application on Wound Heal. 2011;45(4):226–33.

Harmita, Radji M. Buku Ajar Analisis Hayati. 3rd ed. Manurung J, editor. Jakarta: Buku Kedokteran EGC; 2010. 63-68 p.

Zhang L TI. Activation of a mouse macrophage cell line by acemannan: the major carbohydrate fraction from Aloe vera gel. Immunopharmacol.1996;35(2):119–28.

Ramamoorthy L, Tizard IANR. Induction of Apoptosis in a Macrophage Cell Line RAW 264 . 7 By Acemannan , ( 1 , 4 ) -Acetylated Mannan. 1998;421:415–21.

Yildiz K, Ince AT, Gangarapu V, Bugdaci MS, Baysal B, Kayar Y, et al. Evaluation of concentrations of pro/anti-inflammatory cytokines after complication-free ECRP in cholangiocarcinoma.Turkish J Gastroenterol. 2015; 251):133–7.Available from: http://www.turkjgastroenterol.org

Kaur K, Chang HH, Topchyan P, Cook JM, Barkhordarian A, Eibl G, et al. Deficiencies in natural killer cell numbers, expansion, and function at the pre-neoplastic stage of pancreatic cancer by KRAS mutation in the pancreas of obese mice. Front Immunol. 2018;9 (Jun):1–12.